By G.D. Aurbach and Donald B. McCormick (Eds.)
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Bach, unpublished results)]. 7. Site of Synthesis Scott and Baxter (1991) demonstrated that cultured rat hepatocytes synthesized ALS activity. ALS activity was stimulated twofold when hepatocytes were incubated with bovine GH. , 1992). 8. Properties of Rat ALS Several observations suggest that unlike human ALS, rat ALS may bind free IGFBP-3 as well as IGF-I (IGF-II):IGFBP-3binary complexes. Yang et al. (1989)demonstrated that after fractionation of adult rat serum, IGF-I1tracer bound to presumably unoccupied binding sites in the 150-kDa region; these sites had preferential affinity for IGF-11.
Human. Kiefer et al. (1991b) observed strong hybridization to adult human liver, and positive hybridization in human embryonic liver, osteosarcoma, and human brain. Although cDNA clones for human IGFBP-4 were isolated from a placenta library, La Tour et al. (1990) did not observe hybridization to RNA from term placenta. 5. IGFBP-5 mRNA was present at high levels in lung, heart, stomach, adrenal, and large and small intestine, but low in abundance in brain, spleen, liver, and testes. Kiefer et al.
1991) in the interphotoreceptor matrix of the bovine retina also may represent IGFBP-6. 2. cDNA On the basis of the sequence of NH,-terminal and internal peptides of porcine IGFBP-6, Shimasaki et al. (1991a) amplified a DNA fragment from a rat ovary cDNA library and used it to isolate a rat IGFBP-6 cDNA clone, which in turn was used to isolate a human IGFBP-6 cDNA clone from a placenta library. Kiefer et al. (1991b) isolated an IGFBP-6 clone from a human osteosarcoma library. Mature rat and human IGFBP-6 contain 201 and 216 amino acids, respectively, and have molecular masses of 21,461 and 22,847 Da.
Advances in Research and Applications by G.D. Aurbach and Donald B. McCormick (Eds.)