By Jim Murphy
1793, Philadelphia. The nation's capital and the most important urban in North the US is devastated through an it sounds as if incurable ailment, reason unknown . . .
In a strong, dramatic narrative, seriously acclaimed writer Jim Murphy describes the disease often called yellow fever and the toll it took at the city's citizens, concerning the epidemic to the most important social and political occasions of the day and to 18th-century scientific ideals and practices. Drawing on first-hand bills, Murphy spotlights the heroic position of Philadelphia's unfastened blacks in fighting the sickness, and the Constitutional main issue that President Washington confronted while he was once compelled to go away the city—and all his papers—while escaping the lethal contagion. the hunt for the fever's explanations and medication, now not stumbled on for greater than a century later on, presents a suspenseful counterpoint to this riveting real tale of a urban below siege.
An American Plague's a number of awards contain a Sibert Medal, a Newbery Honor, and designation as a countrywide ebook Award Finalist. completely researched, generously illustrated with attention-grabbing archival prints, and unflinching in its dialogue of scientific info, this ebook bargains a glimpse into the stipulations of yank towns on the time of our nation's beginning whereas drawing well timed parallels to modern day epidemics. Bibliography, map, index.
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Extra resources for An American Plague: The True and Terrifying Story of the Yellow Fever Epidemic of 1793
Hybridization, the nitrocellulose is removed and washed in buffers of increasing stringency to remove non-specifically bound probe from noncomplementary DNA sites and from the filter itself. The nitrocellulose is then covered with a sheet of x-ray film to reveal the plaques containing complementary DNA sequences as spots on the x-ray film. To recover phage clones identified by the hybridization procedure, the x-ray film is aligned with the original phage plate. Plaques lying under the autoradiographic spots are removed, and the phage are amplified on plates or in suspension culture.
Pre-mRNAs can have alternative cleavage (and polyadenylation) sites that will result in the inclusion or exclusion of sequence information at the 3' end. An example is the immunoglobulin fl heavy chain (IgM) gene, where alternative cleavage sites are chosen depending on the state of maturation of the immunocyte [133-135]. Cleavage at the downstream site in the resting lymphocyte adds an exon that encodes a sequence of hydrophobic amino acids. This sequence serves as a membrane-spanning domain that anchors the protein on the cell surface, where it acts as a receptor.
E) An ex on may have several ends. An example is fibronectin. The internal exons of primary transcripts can also be alternatively spliced. There are cases where a sequence that functions as an exon in one pre-mRNA molecule is removed in another (e. , aA-crystallin ). , troponin T ). In both of these examples, one RNA's exon can be another's intron, and thus a clear-cut distinction between exons and introns can be somewhat difficult to make. , fibronectin ). This suggests that, rather than being considered absolute entities, exons and introns need to be defined operationally.
An American Plague: The True and Terrifying Story of the Yellow Fever Epidemic of 1793 by Jim Murphy